Simultaneous extraction, derivatisation and analysis of varietal thiols and their non-volatile precursors from beer

Varietal thiols are important contributors to the aroma profile of a wide range beverages including beer. A methodology for simultaneous extraction and subsequent analysis varietal 3-sulfanylhexan-1-ol (3SH), 3-sulfanylhexylacetate (3SHA) 4-methyl-4-sulfanylpentan-2-one (4MSP), non-volatile precursors 3-S-cysteinylhexan-1-ol (Cys-3SH) 3-S-glutathionylhexan-1-ol (GSH-3SH) in beer was developed validated first time. The method, which utilises LC-MS/MS, successfully tested on nine commercial beers, showing it be widely applicable this matrix. syntheses novel internal standards d 8 -3SH -3SHA, allow robust accurate quantification hydronated congeners, also reported. simplicity QuEChERS-based (Quick, Easy, Cheap, Effective, Rugged Safe) allows rapid, high throughput analyses suitable both research industry applications. • QuEChERS protocol extract from Ebselen derivatisation enabled using LC-MS/MS. were analysed single chromatographic run. Method performances applied beers samples.